目的 獲得鮮三七和干三七菌群結(jié)構(gòu)信息。方法 以鮮三七和干三七為研究材料，利用Illumina MiSeq平臺，采用高通量測序并進行數(shù)據(jù)分析。結(jié)果 鮮三七和干三七共獲得637個操作分類單元（OTUs），其中15個OTU為共有，鮮三七和干三七特有的OTU數(shù)量分別為484、123個。新鮮三七中占比較大的菌門為變形菌門（Proteobacteria，59.7%）、厚壁菌門（Firmicutes，40.1%）；干三七中占比較高的菌門為厚壁菌門（Firmicutes，99.8%）。鮮三七中占比較大的菌屬為腸桿菌屬（Enterobacter，37.4%）、嗜堿菌屬（Alkaliphilus，11.5%）；干三七中占比較大的菌屬是芽孢桿菌屬（Bacillus，54.6%），類芽孢桿菌（Paenibaciiius，44.9%）。KEGG功能預(yù)測表明干三七的萜類化合物和聚酮類化合物代謝功能菌群的相對豐度高于鮮三七。結(jié)論 鮮三七菌群多樣性高于干三七；干三七中的萜類化合物和聚酮類化合物代謝功能菌群豐富度高于鮮三七，這為三七發(fā)酵皂苷菌株篩選提供了重要參考。

Objective The purpose of this study was to obtain the bacterial communities structures of fresh and dry Panax notoginseng. Methods The bacterial were sequenced by Illumina MiSeq high-throughput sequencing technology, and the biological information was analyzed. Results A total of 637 OTUs were obtained, of which 15 were in common, 484 were from fresh P. notoginseng and 123 were from dry P. notoginseng respectively. The larger phylum of fresh sample was Proteobacteria (59.7%) and Firmicutes (40.1%) while the dry sample were Firmicutes (99.8%). The main genus of fresh sample was Enterobacter (37.4%) and Alkaliphilus (11.5%), while dry sample were Bacillus (54.6%) and Paenibaciiius (44.9%). The functional prediction of KEGG showed that the population of bacteria metabolizing terpenoids and polyketones of dry sample was higher than that of fresh sample. Conclusion The diversity of microbiota in fresh P. notoginseng was higher than that of dry sample; The species with the metabolic function of interpenoids and polyketones of dry P. notoginseng was higher than that of fresh sample, which provides an important reference for screening the microorganism for biotransformation of saponins in P. notoginseng.

河南省教育廳重點科研項目：三七內(nèi)生菌分離篩選及其在藥渣皂苷生物合成中的應(yīng)用（19A180031）