目的 對(duì)不同產(chǎn)地薏苡仁藥材中的7種甘油三酯含量及2種不飽和脂肪酸含量進(jìn)行測(cè)定，評(píng)價(jià)不同產(chǎn)地薏苡仁藥材的質(zhì)量。方法 采用HPLC-ELSD法測(cè)定薏苡仁藥材中7種甘油三酯含量，建立HPLC-UV法測(cè)定油酸、亞油酸含量，并結(jié)合聚類分析對(duì)薏苡仁藥材質(zhì)量進(jìn)行評(píng)價(jià)。結(jié)果 14批薏苡仁藥材中三亞油酸甘油酯、1，2-二亞油酸-3-油酸甘油酯、1，2-二亞油酸-3-棕櫚酸甘油酯、1，2-二油酸-3-亞油酸甘油酯、1-棕櫚酸-2油酸-3-亞油酸甘油酯、三油酸甘油酯、1，2-二油酸-3-棕櫚酸甘油酯質(zhì)量分?jǐn)?shù)分別在0.31%～0.83%、0.81%～1.81%、0.38%～0.95%、1.19%～2.39%、0.67%～1.58%、0.71%～1.55%、0.44%～1.13%；14批薏苡仁藥材中油酸、亞油酸含量分別在0.45%～0.72%、0.37%～0.53%；聚類結(jié)果可知，以甘油三酯和脂肪酸為變量可以把薏苡仁藥材聚為3類。結(jié)論 不同產(chǎn)地薏苡仁藥材甘油三酯及脂肪酸的含量存在一定的差異，聚類結(jié)果未發(fā)現(xiàn)其含量和產(chǎn)地的相關(guān)性。多組分含量測(cè)定能更全面地反映薏苡仁藥材的質(zhì)量。

Objective To determine the seven kinds of triglycerides and two kinds of unsaturated fatty acids in Coicis Semen in order to evaluate the quality of Coicis Semen in different producing areas. Methods HPLC-ELSD method was used to determine the content of seven triglycerides in Coicis Semen. The HPLC-UV method was established to determine the content of oleic acid and linoleic acid. The cluster analysis method was used for quality evaluation. Results The content of trilinolein, 1,2-dilinoleic acid-3-olein, 1,2-dilinoleic acid-3-palmitin, 1,2-dioleic acid-3-linolein, 1-palmitic acid-2 oleic acid-3-linolein, triolein, 1,2-dioleic acid-3-palmitin were 0.31%-0.83%, 0.81%-1.81%, 0.38%-0.95%, 1.19%-2.39%, 0.67%-1.58%, 0.71%-1.55%, 0.44%-1.13%, respectively. The content of oleic acid and linoleic acid in 14 batches of Coicis Semen were 0.45%-0.72% and 0.37%-0.53%, respectively. Adopting triglyceride and fatty acid as variables, the samples can be grouped into three categories. Conclusion Some differences existed in content of triglyceride and fatty acid in Coicis Semen from different areas, but the clustering results did not find the correlation between its content and origin. The determination of multi-components comprehensively reflected the quality of Coicis Semen.

國(guó)家自然科學(xué)基金資助項(xiàng)目（81873016）；國(guó)家自然科學(xué)基金資助項(xiàng)目（81673606）；江蘇省科教強(qiáng)衛(wèi)醫(yī)學(xué)重點(diǎn)人才項(xiàng)目（ZDRCA2016036）