目的 通過網(wǎng)絡(luò)藥理學(xué)方法研究新型冠狀病毒肺炎（COVID-19）中期診療方（XGZQZLF）治療COVID-19的作用機(jī)制。方法 采用網(wǎng)絡(luò)藥理學(xué)方法，以中藥系統(tǒng)藥理學(xué)數(shù)據(jù)庫、GeneCards數(shù)據(jù)庫等為支撐，通過Cytoscape構(gòu)建“藥物-活性成分-靶點(diǎn)-疾病”網(wǎng)絡(luò)，進(jìn)一步進(jìn)行基因富集分析和通路注釋，研究XGZQZLF治療COVID-19的物質(zhì)基礎(chǔ)和作用機(jī)制。結(jié)果 篩選出XGZQZLF有效成分80個(gè)，通過調(diào)控蛋白激酶（AKT1）、白細(xì)胞介素6（IL-6）、絲裂原活化蛋白激酶3（MAPK3）、血管內(nèi)皮生長因子A（VEGFA）、半胱氨酸蛋白酶3（CASP3）等208個(gè)肺炎相關(guān)靶點(diǎn)，以及主要通過調(diào)控TNF信號通路、IL-17信號通路、Th17細(xì)胞分化、缺氧誘導(dǎo)因子1（HIF-1）信號通路等炎癥和免疫相關(guān)通路發(fā)揮治療COVID-19的作用。結(jié)論 XGZQZLF通過多成分、多靶點(diǎn)、多通路協(xié)同作用發(fā)揮治療COVID-19的作用，為XGZQZLF治療COVID-19的生物機(jī)制研究提供數(shù)據(jù)支撐，同時(shí)為COVID-19藥物研發(fā)提供參考。

Objective To research the mechanism of Xinguanzhongqizhenliao formula (XGZQZLF) for COVID-19 treatment by network pharmacology. Methods "Drug-active ingredient-target-disease" network was constructed by Cytoscape based on TCMSP and GeneCards databases. Mechanism of XGZQZLF in treatment of COVID-19 was implemented by GO and KEGG analysis. Results A total of 80 active ingredients of XGZQZLF were screened out. They could regulate 208 targets of COVID-19, including AKT1, IL-6, MAPK3, VEGFA, CASP3 and so on, which exerted therapeutic effect on COVID-19 through regulating multiple signaling pathways such as TNF signaling pathway, IL-17 signaling pathway, Th17 cell differentiation, HIF-1 signaling pathway and so on. Conclusion XGZQZLF plays an important role in the treatment of COVID-19 through multi-component, multi-target and multi-pathway, which can provide reference for the synergistic theory of TCM compound prescription.

R285.5

國家重大新藥創(chuàng)制項(xiàng)目（2018ZX09201011）；國家中醫(yī)藥管理局名老中醫(yī)藥專家傳承工作室建設(shè)項(xiàng)目；北京中醫(yī)藥大學(xué)創(chuàng)新團(tuán)隊(duì)項(xiàng)目（2019-JYB-TD011）；中華中醫(yī)藥學(xué)會(huì)青年托舉人才項(xiàng)目（QNRC2-A03）