[關(guān)鍵詞]
[摘要]
目的 研究大苞鞘石斛Dendrobium wardianum的主要化學(xué)成分及部分化合物的腫瘤細胞毒活性。方法 應(yīng)用多種色譜分離技術(shù)，結(jié)合半制備液相色譜對化合物進行分離純化，通過核磁共振譜、質(zhì)譜、薄層色譜對照等鑒定化合物結(jié)構(gòu)；MTS法檢測部分化合物的腫瘤細胞毒活性。結(jié)果 從大苞鞘石斛中分離得到16個化合物，分別鑒定為愉悅石斛素（1）、鉤狀石斛素（2）、反式-阿魏酸二十烷基酯（3）、反式-阿魏酸二十二烷基酯（4）、對香豆素酸二十四烷基酯（5）、4-羥基-3-甲氧基肉桂醛（6）、二氫松柏醇（7）、3，7-二甲氧基-5-羥基-1，4-菲醌（8）、3-乙氧基-5-羥基-7-甲氧基-1，4-菲醌（9）、4-甲氧基-9，10-二氫菲-2，7-二醇（10）、5，7，4'-三羥基二氫黃酮（11）、5，7，4'-三羥基-3'，5'-二甲氧基二氫黃酮（12）、苜蓿素（13）、3，5，4'-三甲氧基-4-羥基聯(lián)芐（14）、3，5，3'-三甲氧基-4'-羥基聯(lián)芐（15）和3，3'-二甲氧基-4，5'-二羥基聯(lián)芐（16）。對所得化合物3、6、8、9、11、14、15進行了腫瘤細胞毒活性篩選，其中化合物6、8有一定的體外腫瘤生長抑制活性。結(jié)論 化合物3、5、6為首次從石斛屬植物中分離得到，化合物10、11、14、15為首次從大苞鞘石斛中分離得到；大苞鞘石斛活性成分有待挖掘。
[Key word]
[Abstract]
Objective To study the chemical constituents and cytotoxic activity of Dendrobium wardianum. Methods The compounds were separated and purified by various column chromatography techniques and semi-preparative liquid chromatography, and the structures were then identified by NMR, MS and TLC. And the cytotoxic activity of compounds 3, 6, 8, 9, 11, 14, 15 were detected by MTS assay. Results Sixteen compounds were isolated from D. wardianum. They were identified as amotin (1), aduncin (2), E-ferulic acid eicosyl ester (3), E-ferulic acid docosyl ester (4), tetracosyl p-coumarate (5), 4-hydroxy-3-methoxy cinnamaldehyde (6), dihydroconiferyl alcohol (7), 3,7-dimethoxy-5-hydroxy-1,4-phenanthrenequinone (8), 3-ethoxy-5-hydroxy-7- methoxy-1,4-phenanthrenequinone (9), 4-methoxy-9,10-dihydrophilic-2,7-diol (10), 5,7,4'-trihydroxy dihydroflavone (11), 5,7,4'-trihydroxy-3',5'-dimethoxy dihydroflavone (12), tricin (13), 3,5,4'-trimethoxy-4-hydroxybibenzyl (14), 3,5,3'-trimethoxy-4'-hydroxybibenzyl (15) and 3,3'-dimethoxy-4,5'-dihydroxybibenzyl (16). Compounds 6 and 8 have tumor growth inhibitory activity on tumor growth in vitro. Conclusion Compounds 3, 5, 6 are isolated from Dendrobium for the first time, and 10, 11, 14, 15 are isolated from D. wardianum for the first time. The active components of D. wardianum need to be further explored.
[中圖分類號]
R284.1
[基金項目]
云南省科技專項“云南石斛產(chǎn)業(yè)轉(zhuǎn)型升級技術(shù)研究與產(chǎn)業(yè)化”（2017ZF003-04）
