目的 研究蕓香科黃皮屬植物光滑黃皮Clausena lenis莖中的化學(xué)成分。方法 綜合運(yùn)用硅膠柱色譜、ODS柱色譜、Sephadex LH-20凝膠柱色譜以及制備型高效液相色譜等方法進(jìn)行系統(tǒng)分離和純化，根據(jù)化合物的理化性質(zhì)及其波譜數(shù)據(jù)，并通過(guò)與文獻(xiàn)中報(bào)道的波譜數(shù)據(jù)進(jìn)行對(duì)照，鑒定化合物的化學(xué)結(jié)構(gòu)。結(jié)果 從光滑黃皮莖的90%乙醇提取物中分離得到了18個(gè)化合物，分別鑒定為6，8-diprenylumbelliferone（1）、byakangelicin（2）、tert-O-methylheraclenol（3）、pabularinone（4）、異栓翅芹烯醇（5）、獨(dú)活屬醇（6）、歐前胡素（7）、前胡醇（8）、花椒樹(shù)皮素甲（9）、異紫花前胡內(nèi)酯（10）、茵芋堿（11）、香草木寧（12）、N-metilatanina（13）、clausine Z（14）、九里香堿（15）、2-methoxy-1-（3-methyl-buten-1-yl）-9H-carbazole-3-carbaldehyde（16）、claulansine I（17）和clausehainanine A（18）。其中化合物1～10為香豆素類(lèi)化合物，11～18為生物堿類(lèi)化合物。結(jié)論 所有化合物均為首次從光滑黃皮中分離得到，其中化合物2、3和8為首次從黃皮屬植物中分離得到。

Objective To study the coumarins and alkaloids from the stems of Clausena lenis. Methods The chemical constituents from the stems of C. lenis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as the comparisons with the data reported in literatures. Results A total of 18 compounds were isolated from the 90% ethanol extract of the stems of C. lenis, which were identified as 6,8-diprenylumbelliferone (1), byakangelicin (2), tert-O-methylheraclenol (3), pabularinone (4), isogosferol (5), heraclenol (6), imperatorin (7), decursinol (8), xanthyletin (9), marmesin (10), skimmianine (11), kokusaginine (12), N-metilatanina (13), clausine Z (14), murrayanine (15), 2-methoxy-1-(3-methyl-buten-1-yl)-9H-carbazole-3-carbaldehyde (16), claulansine I (17) and clausehainanine A (18). Among them, compounds 1-10 were coumarins and 11-18 were alkaloids. Conclusion All compounds are isolated from C. lenis for the first time, compounds 2, 3 and 8 are separated from the genus Clausena for the first time.

R284.1

國(guó)家自然科學(xué)基金資助項(xiàng)目（31660097）；國(guó)家自然科學(xué)基金資助項(xiàng)目（21662011）；國(guó)家自然科學(xué)基金資助項(xiàng)目（21967008）；海南省重點(diǎn)研發(fā)計(jì)劃項(xiàng)目（ZDYF2019049）；?？谑兄攸c(diǎn)研發(fā)計(jì)劃項(xiàng)目（2017050）；教育部創(chuàng)新團(tuán)隊(duì)項(xiàng)目（IRT-16R19）