目的 研究墊狀卷柏Selaginella pulvinata全草的化學(xué)成分。方法 采用硅膠、Sephadex LH-20等多種柱色譜方法進(jìn)行分離純化，應(yīng)用理化方法和波譜分析進(jìn)行結(jié)構(gòu)鑒定；采用MTT法測(cè)定化合物對(duì)人非小細(xì)胞肺癌A549細(xì)胞的抑制活性。結(jié)果 從墊狀卷柏全草的95%乙醇提取物的醋酸乙酯部位分離得到1個(gè)新骨架類型的炔酚類化合物，鑒定為9-羥基-3,4,13-三(4-羥基苯基)-7H-二苯并[gh,pqr]四苯基-7-酮（1）；化合物1對(duì)A549細(xì)胞顯示出較強(qiáng)的抑制活性，其半數(shù)抑制濃度（half maximal inhibitory concentration，IC₅₀）為（16.29±1.44）μg/mL。結(jié)論 化合物1為新化合物，命名為卷柏新酮，具有選擇性細(xì)胞毒活性。

Objective To study the chemical constituents of the whole plants of Selaginella pulvinata. Methods The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures of the isolated compounds were identified based on physicochemical properties and spectroscopic data, and the cytotoxic activities against tested human cancer cell lines were evaluated by MTT method. Results A novel selaginellin derivative was isolated from the whole plants of S. pulvinata, which was identified as 9-hydroxy-3,4,13-tris(4-hydroxyphenyl)-7H-dibenzo[gh,pqr]tetraphen-7-one (1). Compound 1 showed potent inhibitory activity against human non-small cell lung cancer A549 cell with its half maximal inhibitory concentration of (16.29 ±1.44) μg/mL. Conclusion Compound 1 is a novel compound, named selaginbenzopyrene A, which shows selective cytotoxic activity.

R284.1

國(guó)家自然科學(xué)基金項(xiàng)目(82073983)