[關(guān)鍵詞]
[摘要]
目的 通過(guò)HPLC指紋圖譜和多成分測(cè)定結(jié)合灰色關(guān)聯(lián)分析研究不同類(lèi)型黃酒炮制黃芩飲片的質(zhì)量,以確定炮制酒黃芩飲片使用的黃酒類(lèi)型。方法 采用HPLC法檢測(cè)10批不同類(lèi)型黃酒炮制的30批酒黃芩飲片樣品中黃芩苷、漢黃芩苷、黃芩素、漢黃芩素、白楊素和千層紙素A的含量并建立其指紋圖譜,測(cè)定30批酒黃芩飲片樣品的總黃酮與醇溶性浸出物含量;結(jié)合聚類(lèi)分析和灰色關(guān)聯(lián)度分析對(duì)30批酒黃芩飲片樣品的質(zhì)量進(jìn)行綜合評(píng)價(jià)。結(jié)果 建立了30批酒黃芩飲片樣品的HPLC指紋圖譜;30批酒黃芩飲片樣品的相對(duì)關(guān)聯(lián)度為0.347 3~0.619 7,飲片樣品質(zhì)量存在差異;黃酒含糖量與酒黃芩飲片樣品質(zhì)量的相關(guān)系數(shù)為0.723,存在一定相關(guān)性。結(jié)論 酒精度數(shù)為15%的干型黃酒(含糖量≤15.0 g/L)制得的酒黃芩飲片質(zhì)量最佳,為酒黃芩飲片炮制提供實(shí)驗(yàn)基礎(chǔ)。
[Key word]
[Abstract]
Objective In order to determine the type of yellow rice wine (Huangjiu) used in the processing of wine Huangqin (Scutellariae Radix, SR), the quality of wine SR pieces prepared by different types of Yellow rice wine was studied by HPLC fingerprint and multi-component determination, combined with grey correlation analysis. Methods The contents of baicalin, wogonoside, baicalein, wogonin, chrysin and oroxylin A in 30 batches of wine SR samples processed with ten different types of Yellow rice wine were detected by HPLC and their fingerprints were established. The contents of total flavonoids and alcohol-soluble extracts in 30 batches of wine SR samples were determined. The quality of 30 batches of samples was comprehensively evaluated by combining cluster analysis and grey correlation analysis. Results The fingerprint of 30 batches of wine SR samples was established. The relative correlation degree of 30 batches of wine SR decoction pieces was between 0.347 3 and 0.619 7, and the quality of samples was different. The correlation coefficient between the sugar content of Yellow rice wine and the quality of wine SR samples was 0.723, and there was a certain correlation. Conclusion The quality of wine-processed SR prepared from dry Yellow rice wine (sugar content ≤ 15.0 g/L) with 15% alcohol content is the best, which provides an experimental basis for the processing of wine SR slices.
[中圖分類(lèi)號(hào)]
R283.6
[基金項(xiàng)目]
國(guó)家重點(diǎn)研發(fā)計(jì)劃子任務(wù)(2021YFD1600903-02);吉林省教育廳科學(xué)技術(shù)研究項(xiàng)目(JJKH20230991KJ);吉林省科技發(fā)展計(jì)劃項(xiàng)目(YDZJ202301ZYTS460);吉林省大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(S202310199019)