目的 篩選刺五加Eleutherococcus senticosus中的PsbP基因（EsPsbP基因），分析其進(jìn)化特征，研究EsPsbP蛋白與PsbQ蛋白的結(jié)合方式，探究其在不同光照下的表達(dá)量。方法 根據(jù)刺五加基因組數(shù)據(jù)篩選得到EsPsbP基因，并對(duì)其進(jìn)行生物信息學(xué)、適應(yīng)性進(jìn)化分析和分子動(dòng)力學(xué)模擬，根據(jù)轉(zhuǎn)錄組測(cè)序結(jié)果探究EsPsbP在不同光照下的表達(dá)量。結(jié)果 共篩選得到了16個(gè)EsPsbP基因并將其分為了A～I(xiàn)共9組。在刺五加種內(nèi)與刺五加和龍牙楤木Aralia elata間共得到了21對(duì)共線性基因?qū)?，并且基因?qū)Φ?i>K_a/K_s均小于1。EsPsbP基因沒有正選擇位點(diǎn)。EsPsbP基因啟動(dòng)子中存在大量光響應(yīng)元件、激素響應(yīng)元件和干旱響應(yīng)元件。分子動(dòng)力學(xué)模擬發(fā)現(xiàn)PsbQ蛋白優(yōu)先與A組的EsPsbP蛋白進(jìn)行結(jié)合。轉(zhuǎn)錄組數(shù)據(jù)顯示，不同光質(zhì)照射后，有9個(gè)EsPsbP產(chǎn)生了差異表達(dá)。結(jié)論 刺五加的16條EsPsbP基因在進(jìn)化中主要受到純凈選擇，這有助于維持基因的穩(wěn)定性?；蚪Y(jié)構(gòu)特征的分化可能導(dǎo)致EsPsbP與PsbQ蛋白結(jié)合能力及對(duì)光質(zhì)變化反應(yīng)的差異，這些特性可能與刺五加的生理適應(yīng)及藥材品質(zhì)形成相關(guān)，為進(jìn)一步研究其功能奠定了基礎(chǔ)。

Objective To screen the EsPsbP gene in Eleutherococcus senticosus, analyse its evolutionary characteristics, study the binding mode of EsPsbP protein and PsbQ protein, and explore its expression under different light.Methods The EsPsbP genes were screened and obtained based on the genomic data of E. senticosus and were subjected to bioinformatics, adaptive evolutionary analyses and molecular dynamics simulations, and the expression of EsPsbP under different light conditions was investigated based on the results of transcriptome sequencing. Results A total of 16 EsPsbP genes were screened and classified into nine groups, A—I. A total of 21 covariate gene pairs were obtained within E. senticosus and between E. senticosus and Aralia elata, and the K_a/K_s of the gene pairs were all less than 1. There was no positively selected site in EsPsbP gene, and a large number of light-responsive, hormone-responsive, and drought-responsive elements existed in the promoter of EsPsbP gene. Molecular dynamics simulations revealed that PsbQ proteins preferentially bound to EsPsbP proteins in group A. Transcriptomic data showed that nine EsPsbPs were differentially expressed after different light quality irradiation. Conclusion A total of 16 EsPsbP genes of E. senticosus have been subjected to predominantly pure selection during evolution, which has helped to maintain gene stability. The divergence of structural features of the genes may lead to differences in the binding capacity of EsPsbP and PsbQ proteins and the response to light quality changes, and these characteristics may be related to the physiological adaptations of E. senticosus and the formation of medicinal herb quality, which lays the foundation for further research on its function.

R282.12

國(guó)家自然科學(xué)基金資助項(xiàng)目(32470398);河北省中央引導(dǎo)地方科技發(fā)展資金(236Z2501G);河北省自然科學(xué)基金生物醫(yī)藥聯(lián)合基金(H2020209302)