目的 建立不同來源茵陳Artemisiae Scopariae Herba藥材的UPLC多成分含量測(cè)定和指紋圖譜方法，對(duì)28個(gè)產(chǎn)地綿茵陳和22個(gè)產(chǎn)地花茵陳藥材進(jìn)行含量測(cè)定、指紋圖譜分析和化學(xué)模式分析。方法 以Waters ACQUITY UPLC BEH C₁₈（100 mm×2.1 mm，1.7 µm）色譜柱為固定相，0.1%甲酸水（A）­乙腈（B）為流動(dòng)相進(jìn)行梯度洗脫，體積流量0.25 mL/min，檢測(cè)波長(zhǎng)310 nm，結(jié)合對(duì)照品比對(duì)進(jìn)行色譜峰確認(rèn)，對(duì)共50份不同來源茵陳藥材進(jìn)行14個(gè)化合物的含量測(cè)定，指紋圖譜分析共有峰和進(jìn)行相似度評(píng)價(jià)，采用聚類分析（hierarchical cluster analysis，HCA）和正交偏最小二乘法判別分析（orthogonal partial least squares discrimination analysis，OPLS-DA）篩選茵陳藥材的特征成分。結(jié)果 建立了茵陳藥材UPLC多成分含量測(cè)定和指紋圖譜方法；結(jié)果表明，不同產(chǎn)地來源茵陳化學(xué)成分含量存在差異；指紋圖譜法標(biāo)定了綿茵陳、花茵陳各18、32個(gè)共有峰，化學(xué)模式分析表明化合物3,4-二咖啡酰奎寧酸、3,5-二咖啡?？鼘幩岷?,5-二咖啡?？鼘幩峥勺鳛樗幉馁|(zhì)量評(píng)價(jià)的標(biāo)志性成分。結(jié)論 建立的UPLC多成分含量測(cè)定方法和指紋圖譜法適用于不同來源茵陳的質(zhì)量評(píng)價(jià)；不同產(chǎn)地的含量測(cè)定結(jié)果、指紋圖譜和化學(xué)模式分析結(jié)果可為茵陳藥材產(chǎn)地選擇、質(zhì)量控制提供有益參考。

Objective Establishing UPLC multi-component content determination and fingerprint analysis methods for Yinchen (Artemisiae Scopariae Herba) from different sources. Determination the content, fingerprint analysis and chemical pattern analysis of Artemisiae Scopariae Herba (Mian Yinchen) and Artemisiae Scopariae Herba (Hua Yinchen) were conducted from 28 origins and 22 origins, respectively. Methods Using Waters ACQUITY UPLC BEH C₁₈ (100 mm×2.1 mm, 1.7 µm) column as the stationary phase, 0.1% formic acid water (A)-acetonitrile (B) was used as the mobile phase for gradient elution, with a flow rate of 0.25 mL/min and a detection wavelength of 310 nm. The chromatographic peaks were confirmed by comparing with reference materials. A total of 50 different sources of Artemisiae Scopariae Herba were quantitatively determined for 14 compounds, and fingerprint analysis was performed to evaluate common peaks and similarity evaluation. Hierarchical cluster analysis and orthogonal partial least squares discrimination analysis (HCA and OPLS-DA) were used to screen the characteristic components of Artemisiae Scopariae Herba. Results Establishing a UPLC multi-component content determination and fingerprint method for Artemisiae Scopariae Herba; The quantitative analysis results indicated that there are differences in the chemical composition content of Artemisiae Scopariae Herba from different regions; The fingerprint method calibrated 18 and 32 common peaks for two harvesting periods, and chemical pattern analysis showed that 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid and 4,5-dicaffeoyl quinic acid can be used as landmark components for quality evaluation of medicinal materials. Conclusion The established UPLC multi-component content determination method and fingerprint method are suitable for quality evaluation of Artemisiae Scopariae Herba from different sources; The content determination results, fingerprint spectra, and chemical pattern analysis results from different origins can provide useful references for the selection of origin and quality control of Artemisiae Scopariae Herba.

R286.2

國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2022YFC3501500);中國(guó)醫(yī)學(xué)科學(xué)院醫(yī)學(xué)與健康科技創(chuàng)新工程(2021-I2M-1-032);國(guó)家中醫(yī)藥管理局委托項(xiàng)目(GZY-KJS-2023-008);漳州市名老藥工黃進(jìn)明工作室專項(xiàng)資金項(xiàng)目