目的 研究安息香Benzoinum化學(xué)成分以及體外抑制腫瘤增殖活性。方法 采用MCI gel CHP-20P、正相硅膠、ODS和Sephadex LH-20柱色譜以及半制備型高效液相等色譜方法進行分離和純化，通過紅外、核磁共振、質(zhì)譜等技術(shù)鑒定化合物的結(jié)構(gòu)。CCK-8法評價分離得到的化合物體外抑制腫瘤增殖的活性。結(jié)果 從安息香醋酸乙酯萃取部位中分離得到3個化合物，分別鑒定為1,3-二苯甲酸酯-2-(羥基(4-羥基-3-甲氧基苯基)甲基)丙烷（1）、5-乙?；?7-甲氧基-2-(3,4-亞甲二氧基苯基)苯駢呋喃（2）和5-甲?；?7-甲氧基-2-(3,4-亞甲二氧基苯基)苯駢呋喃（3）?；衔?b>2和3對人肝癌HepG2細胞增殖有抑制作用，半數(shù)抑制濃度（half maximal inhibitory concentration，IC₅₀）值分別為41.2、52.6μmol/L。結(jié)論 化合物1和2為新化合物，命名為白花樹醇酯（1）和白花樹醇酮（2）；化合物3為新的天然產(chǎn)物；其中化合物2和3對人肝癌HepG2細胞增殖表現(xiàn)出一定的細胞毒活性。

Objective To study the chemical constituents from Benzoinum and their antitumor activities in vitro. Methods The compounds were isolated and purified by MCI gel CHP-20P, silica gel, ODS, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were elucidated by spectroscopic analysis including IR, NMR and MS. CCK-8 assay was used to evaluate the inhibitory activity of the isolated compounds on tumor proliferation in vitro. Results A total of three compounds, 1,3-dibenzoate-2-(hydroxy(4-hydroxy-3-methoxyphenyl) methyl)propane (1), 5-acetyl-7-methoxy-2-(3,4-methylenedioxyphenyl) benzofuran (2), and 5-formyl-7-methoxy-2-(3,4-methylenedioxyphenyl)benzofuran (3), were isolated from the ethyl acetate extraction site of Benzoinum. Compounds 2 and 3 exhibited the inhibitory effects on HepG-2 cells with half maximal inhibitory concentration (IC₅₀) values of 41.2, 52.6 μmol·L⁻¹, respectively. Conclusion Compounds 1 and 2 are new compounds named styratonate (1) and styratonone (2), and compound 3 is a new natural product, among which compounds 2 and 3 exhibit certain cytotoxic activities on the proliferation of HepG2 cells.

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江漢大學(xué)科學(xué)專項基金資助項目（2023KYZX28）;廣東省教育廳普通高校重點科研平臺和項目（2022ZDZX2035）