目的 研究不同根際促生菌（plant growth-promoting rhizobacteria，PGPR）對白花前胡Peucedanum praeruptorum根際土壤中磷、鉀元素含量、形態(tài)轉(zhuǎn)變、元素有效性及其根部藥用成分含量的影響，為白花前胡的品質(zhì)培優(yōu)提供參考。方法 通過盆栽接種實驗，將9種不同類型PGPR菌株接種于白花前胡根際土壤，試驗設(shè)為空白對照（CK）、接種Bacillus thuringiensis（S1）、Paenibacillus amylolyticus（S2）、B. polymyxa（S3）、B. aryabhattai（S4）、B. aryabhattai（S5）、B. cereus（S6）、B. mycoides（S7）、B. proteolyticu（S8）和B. wiedmannii（S9）10個處理組；共生培養(yǎng)9個月后，測定根際土壤中不同形態(tài)磷、鉀元素及根部中白花前胡甲素、白花前胡乙素含量，分析接種PGPR后白花前胡根部藥用成分與根際土壤磷、鉀形態(tài)含量的變化，并通過相關(guān)性與通徑分析探討白花前胡有效成分與根際土壤磷、鉀形態(tài)的相關(guān)性。結(jié)果 與CK組相比，接種PGPR的9個處理組的白花前胡甲素、白花前胡乙素含量均顯著提高，且以S2組效果最為顯著，分別較CK組增長了0.513%、0.649%；接種不同PGPR均不同程度增加了白花前胡根際土壤有機磷、有效磷、全磷以及各形態(tài)無機磷含量，其中以S5組效果最為顯著；接種PGPR可以提高白花前胡根際土壤全鉀以及各形態(tài)鉀素含量，且以S1組效果最為顯著；相關(guān)性分析表明，各形態(tài)無機磷呈極顯著正相關(guān)，F(xiàn)e-P、全磷與有效磷呈顯著性正相關(guān)；全鉀、礦物鉀、水溶鉀均與速效鉀呈極顯著正相關(guān)，緩效鉀與速效鉀呈顯著性正相關(guān)，各鉀素形態(tài)之間呈極顯著正相關(guān)。通徑分析表明，F(xiàn)e-P、緩效鉀對白花前胡甲素呈正效應(yīng)，有機磷、Ca₈-P、有效磷、速效鉀、交換鉀對白花前胡乙素呈正效應(yīng)。結(jié)論 接種不同PGPR能夠有效提高白花前胡根際土壤各形態(tài)磷、鉀素含量，以及白花前胡品質(zhì)和藥用價值，其中以Paenibacillus amylolyticus菌株接種效果最佳。

Objectives To study the effect of different plant growth-promoting Rhizobacteria (PGPR) on the content, transformation, and availability of phosphorus and potassium in the rhizosphere soil of Peucedanum praeruptorum, as well as on the quality of its medicinal components, and to provide a reference for optimizing its cultivation. Methods P. praeruptorum was planted in pots and inoculated with nine different PGPR strains. The contents of various forms of phosphorus and potassium in the rhizosphere soil were measured.The experiment was set up as a control (CK), inoculated with Bacillus thuringiensis (S1), Paenibacillus amylolyticus (S2), B. polymyxa (S3), B. aryabhattai (S4), B. aryabhattai (S5), B. cereus (S6), B. mycoides (S7), B. proteolyticu (S8), and B. wiedmannii (S9) ten treatment groups. After a year of inoculation, the transition between different types of phosphorus and potassium element in the rhizosphere soil as well as the contents of praeruptorin A and praeruptorin B in the roots of P. praeruptorum inoculated with PGPR were determined, and the correlation between medicinal ingredients and the forms of phosphorus and potassium element through correlation analysis and path analysis were discussed. Results Compared with CK group, the nine PGPR inoculation groups significantly increased the content of praeruptorin A and praeruptorin B, and the effect of S2 group was the most significant with the increase of praeruptorin A and praeruptorin B by 0.513% and 0.649% respectively. Inoculation with different PGPRs increased the contents of organic phosphorus, available phosphorus, total phosphorus, and various forms of inorganic phosphorus in the rhizosphere soil of P. praeruptorum in varying degrees, and the effect of S5 group was the most significant. Inoculation with PGPRs also increased the contents of total potassium and various types of potassium in the rhizosphere soil of P. praeruptorum, and the effect of S1 group is the most significant. The correlation analysis showed that there was a significant positive correlation between all forms of inorganic phosphorus, Fe-P, total phosphorus and available phosphorus. Total potassium, mineral potassium and water-soluble potassium were significantly positively correlated with available potassium, while slowly available potassium was significantly positively correlated with available potassium, and the correlation between different forms of potassium were also significantly positive. Path analysis showed that Fe-P and slowly available potassium had a positive effect on praeruptorin A, while organic phosphorus, Ca₈-P, available phosphorus, fast available potassium and exchangeable potassium had a positive effect on praeruptorin B. Conclusions Inoculation with different PGPRs can effectively improve the content of various forms of phosphorus and potassium in the rhizosphere soil of P. praeruptorum, as well as the quality and medicinal value of P. praeruptorum. Among the nine PGPRs, inoculation with P. amylolyticus strain was the most effective.

R286.2

重慶市教委科學技術(shù)研究計劃項目（KJQN202001205）