目的 研究三七Panax notoginseng根莖中的黃酮類成分及其抗血小板聚集活性。方法 采用AB-8大孔樹脂、硅膠、ODS柱色譜以及制備型高效液相色譜等分離技術(shù)對三七根莖中黃酮類成分進(jìn)行分離純化，運(yùn)用核磁共振、質(zhì)譜等波譜技術(shù)和化學(xué)方法對分離的化合物進(jìn)行結(jié)構(gòu)鑒定，并對分離得到的化合物進(jìn)行抗血小板聚集活性評價(jià)。結(jié)果 從三七根莖中分離得到8個(gè)黃酮類化合物，分別鑒定為槲皮素-3-O-β-D-木糖基-(1→2)-β-D-半乳糖基-3'-O-β-D-葡萄糖苷（1）、槲皮素-3-O-β-D-葡萄糖基-(1→2)-β-D-半乳糖苷（2）、槲皮素-3-O-β-D-木糖基-(1→2)-β-D-半乳糖苷（3）、山柰酚-3-O-β-D-葡萄糖基-(1→2)-β-D-半乳糖苷（4）、山柰酚-3-O-β-D-木糖基-(1→2)-β-D-半乳糖苷（5）、異鼠李素-3-O-β-D-木糖基-(1→2)-β-D-半乳糖苷（6）、槲皮素-3-O-β-D-半乳糖苷（7）、槲皮素-3-O-β-D-葡萄糖苷（8）。結(jié)論 化合物1為新化合物，命名為三七黃酮苷；化合物6的波譜數(shù)據(jù)為首次報(bào)道，化合物5和6為首次從五加科中分離得到，化合物7和8為首次從三七中分離得到。發(fā)現(xiàn)三七中黃酮苷的C-3位直接相連的六碳糖為半乳糖是其生物合成的特異性。體外抗血小板聚集測定結(jié)果表明化合物1～7均具有一定的抗血小板聚集活性。

Objective To study the flavonoids in the rhizomes of Panax notoginseng and their antiplatelet aggregation activity. Methods AB-8 macroporous resin, silica gel, ODS column chromatography and preparative high performance liquid chromatography were used to separate and purify the flavonoids in the rhizomes of P. notoginseng. Their structures were elucidated by spectroscopic and chemical analysis, especially NMR and MS. Additionally, the antiplatelet aggregation activity of the isolated compounds was evaluated. Results Eight flavonoids were isolated and identified as quercetin-3-O-β-D-xylosyl-(1→2)-β-D-galactosyl-3'-O-β-D-glucoside (1), quercetin-3-O-β-D-glucosyl-(1→2)-β-D-galactoside (2), quercetin-3-O-β-D-xylosyl-(1→2)-β-D-galactoside (3), kaempferol-3-O-β-D-glucosyl-(1→2)-β-D-galactoside (4), kaempferol-3-O-β-D-xylosyl-(1→2)-β-D-galactoside (5), isorhamnetin-3-O-β-D-xylosyl-(1→2)-β-D-galactoside (6), quercetin-3-O-β-D-galactoside (7), quercetin-3-O-β-D-glucoside (8). Conclusion Compound 1 is a new flavonoid named notoginsenoflavoside. The spectral data for compound 6 were reported for the first time. Moreover, compounds 5 and 6 were isolated from Araliaceae family for the first time, whereas compounds 7 and 8 were isolated from P. notoginseng for the first time. The specificity of flavonoids biosynthesis in P. notoginseng was firstly discovered. Specifically, the hexose directly connected to the C-3 position of flavonoid was galactose. The in vitro anti-platelet aggregation assay indicated that compounds 1~7 exhibited anti-platelet aggregation activity with different inhibition ratios.

R284.1

國家自然科學(xué)基金項(xiàng)目（81703658）;遼寧省教育廳科研平臺(tái)建設(shè)項(xiàng)目（LJ232410162066）;遼寧省科技廳聯(lián)合基金面上項(xiàng)目（2024-MSLH-291）