目的 基于“辨色論質(zhì)”理論對箭葉淫羊藿Epimedium sagittatum葉片色澤與其淫羊藿苷類有效成分進(jìn)行關(guān)聯(lián)分析，為箭葉淫羊藿“辨色論質(zhì)”質(zhì)量評價體系的建立提供理論參考。方法 使用色差法測定103批箭葉淫羊藿葉片表觀色澤指標(biāo)（明度值L^*、紅綠度值a^*、黃藍(lán)度值b^*、總色差E^*）；HPLC法測定葉片中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、寶藿苷I含量；紫外分光光度法測定葉片中總黃酮含量；運用SPSS軟件進(jìn)行差異分析、K-聚類分析、相關(guān)性分析、回歸分析。結(jié)果 103批箭葉淫羊藿葉片L^*為33.38～48.22、a^*為−7.56～−3.50、b^*為9.17～28.68、E^*為36.82～56.38，可見其表觀色澤有顯著差異；其有效成分定量分析顯示，淫羊藿葉片中朝藿定A質(zhì)量分?jǐn)?shù)為0.28～3.55 mg/g、朝藿定B為0.20～3.60 mg/g、朝藿定C為11.10～124.51 mg/g、淫羊藿苷為0.28～30.55 mg/g、寶藿苷I為0.28～7.32 mg/g、總黃酮為26.72～169.12 mg/g；經(jīng)K-聚類分析將箭葉淫羊藿葉片分為Ⅰ～Ⅴ類，其中第Ⅲ類葉片整體色差均一，呈現(xiàn)亮黃綠色稀黃色斑點，有效成分含量相較于其他4類處于最高水平；經(jīng)相關(guān)性分析表明總黃酮和其他5種有效成分含量與L^*、b^*、E^*呈顯著正相關(guān)（P＜0.05），與a^*呈顯著負(fù)相關(guān)（P＜0.05）；經(jīng)回歸分析得淫羊藿苷與色澤指標(biāo)的回歸方程為Y＝0.009 269＋0.000 236L^*＋0.000 881a^*＋0.000 214b^*。結(jié)論 箭葉淫羊藿葉片色澤與有效成分含量高度關(guān)聯(lián)，可為箭葉淫羊藿葉片有效成分含量快速預(yù)測及“辨色論質(zhì)”評價體系的構(gòu)建提供參考依據(jù)。

Objective Based on the theory of “quality discrimination by color characteristic”, the correlation between the leaves color and the effective components of icariin was analyzed in order to provide theoretical reference for the establishment of the quality evaluation system of “quality discrimination by color characteristic” of Epimedium sagittatum. Methods The apparent color indexes (brightness value L^*, redness value a^*, yellowness chromaticity value b^*, and total color difference E^*) of 103 batches of E. sagittatum leaves were determined by the color difference method. The contents of epimedin A, epimedin B, epimedin C, icariin and baohuoside I were determined by HPLC and the total flavonoid contents were determined by UV spectrophotometry in the leaves. Analysis of variance, K-cluster analysis, correlation analysis, and regression analysis were performed by SPSS software. Results The apparent color indexes of L^*, a^*, b^* and E^*of 103 batches of E. sagittatum leaves were in the range of 33.38—48.22, −7.56—−3.50, 9.17—28.68, and 36.82—56.38, respectively, which showed that there was a significant difference in their apparent coloration; Quantitative analysis of the effective components showed that the content of epimedin A, epimedin B, epimedin C, icariin, baohuoside I, total flavonoids in the leaves of E. sagittatum was in the range of 0.28—3.55 mg/g, 0.20—3.60 mg/g, 11.10—124.51 mg/g, ranging from 0.28—30.55 mg/g, 0.28—7.32 mg/g, and ranged from 26.72—169.12 mg/g, respectively. After K-cluster analysis, the leaves of E. sagittatum were divided into Ⅰ—Ⅴ types, in which the leaves of the Ⅲ type were of uniform color, showing bright yellow-green and diluted yellow spots, and the content of active ingredients was at the highest level compared with the other four types. The correlation analysis showed that the total flavonoids and the other five active ingredients were positively correlated with L^*, b^*, and E^* (P < 0.05), and were negatively correlated with a^* (P < 0.05); According to the regression analysis, the regression equation between icariin and color index was Y = 0.009 269 + 0.00 0236L^* + 0.000 881a^* + 0.000 214b^*.Conclusion The results of this study show that the color of the leaves of E. sagittatum is highly correlated with the content of active ingredients, which can provide a reference basis for the rapid prediction of the content of active ingredients in the leaves of E. sagittatum and the construction of the evaluation system of “quality discrimination by color characteristic”.

R286.2

中國博士后科學(xué)基金特別資助項目（2014T170252）;國家自然科學(xué)基金資助項目（82104329，32401226）;河南省重點研發(fā)專項（231111312700，241111310200）;河南省國際科技合作項目（242102521056）;河南省科技攻關(guān)項目（242102310549）