目的 研究粗糠柴Mallotus philippensis果實的化學(xué)成分。方法 采用硅膠、ODS、凝膠及半制備高效液相等色譜分離技術(shù)進行分離純化，通過質(zhì)譜、核磁共振波譜及圓二色譜等方法對化合物進行結(jié)構(gòu)鑒定，采用肉湯微量稀釋法評價化合物的抗菌活性。結(jié)果 從粗糠柴果實的甲醇提取物中分離得到6個化合物，分別鑒定為粗糠柴黃酮P（1）、粗糠柴黃酮Q（2）、5-羥基-7-甲氧基二氫黃酮（3）、(2S)-7-hydroxy-5-methoxy-8-prenylflavanone（4）、(2R,3S,4R)-2,3,4-trimethyl-3-[(3E,7E,11E)-3,8,12,16-tetramethyl-3,7,11,15-heptadecatetraen-1-yl]cyclohexanone（5）和豆甾-4-烯-3-酮（6）?；衔?b>1對新型隱球菌的最低抑菌濃度（minimum inhibitory concentration，MIC）值為128 μg/mL。結(jié)論 化合物1和2為新化合物，化合物5為新天然產(chǎn)物，化合物3和4為首次從大戟科植物中分離得到，化合物6為首次從野桐屬植物中分離得到?；衔?b>1對新型隱球菌有一定的抑制作用。

Objective To investigate the chemical constituents of the fruit of Mallotus philippensis. Methods The compounds were separated and purified by a variety of chromatographic methods including silica gel ODS, gel and semi-preparative high performance liquid chromatography. Their structures were established based on mass spectrometry, nuclear magnetic resonance spectroscopy and circular dichroism. The antimicrobial activities of these isolates were evaluated by broth microdilution method. Results A total of six compounds were obtained from the methanol extract of the fruit of M. philippensis and identified as mallophilol P (1), mallophilol Q (2), 5-hydroxy-7-methoxyflavanone (3), (2S)-7-hydroxy-5-methoxy-8-prenylflavanone (4), (2R,3S,4R)-2,3,4-trimethyl-3-[(3E,7E,11E)-3,8,12,16-tetramethyl-3,7,11,15-heptadecatetraen-1-yl]cyclohexanone (5) and stigmast-4-en-3-one (6), respectively. The minimum inhibitory concentration (MIC) value of compound 1 against Cryptococcus neoformans was 128 μg/mL. Conclusion Compounds 1 and 2 are new compounds, compound 5 is a new natural product, compounds 3 and 4 are reported from the Euphorbiaceae plants for the first time, and compound 6 is reported from the genus Mallotus for the first time. Compound 1 shows certain antimicrobial effect against C. neoformans.

R284.1

國家自然科學(xué)基金資助項目（82373751）；深圳市博士后留深資助項目