目的 研究湖北金粟蘭Chloranthus henryi var. hupehensis的化學(xué)成分及其抗炎活性。方法 采用硅膠、凝膠、HPLC等色譜技術(shù)進(jìn)行提取分離與純化，運(yùn)用MS、NMR、ECD等波譜方法并結(jié)合文獻(xiàn)數(shù)據(jù)，對(duì)化合物結(jié)構(gòu)進(jìn)行鑒定，采用脂多糖（lipopolysaccharides，LPS）誘導(dǎo)的鼠巨噬細(xì)胞RAW 264.7體外炎癥模型進(jìn)行抗炎活性評(píng)價(jià)。結(jié)果 從該植物根部分離得到11個(gè)倍半萜類化合物，分別鑒定為4β,5α,9α-trihydroxy-7-oxoguaia-8(11)-en-12,9-olid（1）、8S,9S-環(huán)氧化白術(shù)內(nèi)酯II（2）、6β,10α-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide（3）、長(zhǎng)尾粗葉木內(nèi)酯A（4）、6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide（5）、白術(shù)內(nèi)酯III（6）、chlorantene C（7）、chlomultin B（8）、dihydrocurcolone（9）、commiphorene B（10）、5β(H)-10α-methyl-8-oxoeudesma-4(15),7(11)-dien-12,6-olide（11）。結(jié)論 化合物1為新的愈創(chuàng)木烷型倍半萜，命名為湖北金粟蘭內(nèi)酯N，化合物2～3、9～11為首次從金粟蘭屬植物中分離得到，化合物1～3、6～11為首次從湖北金粟蘭中分離得到?；衔?b>2對(duì)LPS誘導(dǎo)的鼠巨噬細(xì)胞RAW 264.7的一氧化氮（NO）產(chǎn)生有一定抑制作用，半數(shù)抑制濃度（median inhibition concentration，IC₅₀）為（29.0±11.3）μmol/L。

Objective To study the chemical constituents and anti-inflammatory activities of Chloranthus henryi var. hupehensis. Methods The compounds were extracted, isolated, and purified by column chromatography of silica gel, gel and HPLC, and their structures were identified using spectroscopic methods such as MS, NMR, and ECD combined with literature data. The in vitro anti-inflammatory activities of all compounds were evaluated using the lipopolysaccharide (LPS) induced inflammatory model of RAW 264.7 mouse macrophage cells. Results A total of 11 sesquiterpenoids were isolated from the roots of C. henryi var. hupehensis, which were identified as 4β,5α,9α-trihydroxy-7-oxoguaia-8(11)-en-12,9-olid (1), 8S,9S-epoxylatractylenolide II (2), 6β,10α-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide (3), lasianthuslactone A (4), 6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide (5), atractylenolide Ⅲ (6), chlorantene C (7), chlomultin B (8), dihydrocurcolone (9), commiphorene B (10), 5β(H)-10α-methyl-8-oxoeudesma-4(15),7(11)-dien-12,6-olide (11). Conclusion Compound 1 was a new guaiane-type sesquiterpene named chlorahupelactone N, while compounds 2—3, 9—11 were isolated from Chloranthus genus for the first time, and compounds 1—3, 6—11 were isolated from C. henryi var. hupehensis for the first time. Compound 2 inhibited the production of nitric oxide (NO) in LPS-induced RAW 264.7 mouse macrophage cells with a median inhibition concentration (IC₅₀) value of (29.0 ± 11.3) μmol/L.

R284.1

河北省自然科學(xué)基金資助項(xiàng)目（H2022209046）；中央引導(dǎo)地方科技發(fā)展基金項(xiàng)目（246Z2506G）；南京市中醫(yī)藥科技專項(xiàng)基金資助項(xiàng)目（ZYYB202203）