[關(guān)鍵詞]
[摘要]
目的 為探知天冬Asparagus cochinchinensis塊根生長膨大的分子機(jī)制,對(duì)不同膨大期的天冬進(jìn)行轉(zhuǎn)錄組測序,挖掘促使塊根生長膨大的關(guān)鍵基因。方法 以不同膨大期的天冬為材料,采用Illumina HiSeq高通量測序平臺(tái)進(jìn)行轉(zhuǎn)錄組測序,組裝與注釋后進(jìn)行差異表達(dá)基因(differential gene,DEGs)篩選。結(jié)果 轉(zhuǎn)錄組測序共獲得59.20 Gb clean data,Q30均不小于94.23%,共得到99 930條Unigene,其中47 857條被注釋。3種不同膨大狀態(tài)的天冬中的DEGs有1 429個(gè),GO富集結(jié)果表明,3種不同膨大期天冬的DEGs主要富集于細(xì)胞過程、代謝過程、結(jié)合力與催化活性等功能,同源蛋白簇(clusters of orthologous groups,COG)富集結(jié)果表明,3種不同膨大期的天冬中DEGs主要富集于信號(hào)轉(zhuǎn)導(dǎo)機(jī)制、碳水化合物轉(zhuǎn)運(yùn)代謝以及細(xì)胞壁/膜/被膜的生物合成。京都基因與基因組百科全書(Kyoto encyclopedia of genes and genomes,KEGG)富集分析顯示,與根膨大相關(guān)的通路[植物激素信號(hào)傳導(dǎo)、絲裂原活化蛋白激酶(mitogen-activated protein kinases,MAPK)信號(hào)通路、淀粉與蔗糖代謝以及苯丙烷生物合成]為DEGs代表性富集通路。結(jié)合差異基因的注釋信息以及表達(dá)量情況分析表明,多種酶類(SAUR、XTH、LRR-RLPK、SPP、EG、BGLU、TPP、AMY、BAMY、PAL、PER)參與天冬塊根膨大,其中XTH可能是影響天冬塊根的膨大發(fā)育過程的最主要的酶類。結(jié)論 通過對(duì)不同膨大狀態(tài)天冬的高通量轉(zhuǎn)錄組測序,揭示了天冬塊根生長膨大的關(guān)鍵酶基因,可為天冬根系生長膨大的生物學(xué)機(jī)制提供參考。
[Key word]
[Abstract]
Objective The underground expanded tuberous roots of Asparagus cochinchinensis are used as the herbal medicine Tiandong (Asparagi Radix). In order to investigate the molecular mechanism of A. cochinchinensis tuber growth and expansion, transcriptome sequencing of A. cochinchinensis at different expansion stages was carried out, and the key genes contributing to tuber growth and expansion were excavated. Methods A. cochinchinensis at different expansion stages were used as materials for transcriptome sequencing using Illumina HiSeq high-throughput sequencing platform, and screening of differentially expressed genes was performed after assembly and annotation. Results A total of 59.20 Gb clean data were obtained from transcriptome sequencing, and none of the Q30 was less than 94.23%, and a total of 99 930 Unigenes were obtained, of which 47 857 were annotated. There were 1 429 DEGs in the three different size states of A. cochinchinensis, and GO enrichment results showed that DEGs in asparagus of three different expansion stages were mainly enriched in cellular processes, metabolic processes, and functions such as binding and catalytic activities, and the COG enrichment results showed that DEGs in asparagus of three different expansion stages were mainly enriched in the signal transduction mechanism, carbohydrate transport metabolism, and biosynthesis of cell wall/membrane/transparenchyme, KEGG enrichment analysis showed that the pathways related to root expansion (Plant hormone signal transduction, MAPK signaling pathway-plant, Starch and sucrose metabolism, and Phenylpropanoid biosynthesis) were the main pathways enriched in DEGs. Combined with the annotation information of the differential genes and the analysis of the expression profiles showed that a variety of enzymes (SAUR, XTH, LRR-RLPK, SPP, EG, BGLU, TPP, AMY, BAMY, PAL, PER) are involved in A. cochinchinensis root tuber expansion, among which XTH may be the most important enzyme affecting the expansion and development process. Conclusion By high-throughput transcriptome sequencing of different sizes of A. cochinchinensis, key genes involved in root growth and enlargement were revealed, which can provide reference for the biological mechanism of root growth and enlargement in A. cochinchinensis.
[中圖分類號(hào)]
R286.12
[基金項(xiàng)目]
四川藥品監(jiān)督管理局科技計(jì)劃項(xiàng)目(2023MS615)