目的 建立不同產(chǎn)地批次大青葉Isatidis Folium的HPLC指紋圖譜及指標(biāo)成分含量測(cè)定方法，結(jié)合化學(xué)模式識(shí)別法評(píng)價(jià)不同產(chǎn)地大青葉質(zhì)量。方法 建立大青葉HPLC指紋圖譜，應(yīng)用中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)（2012.130723版本）、IBM SPSS Statistics 27.0、SIMCA14.1軟件，結(jié)合聚類分析（hierarchical cluster analysis，HCA）、主成分分析（principal component analysis，PCA）、正交偏最小二乘法-判別分析（orthogonal partial least squares discrimination analysis，OPLS-DA）對(duì)不同產(chǎn)地大青葉質(zhì)量進(jìn)行評(píng)價(jià)，根據(jù)變量重要性投影（variable importance for projection，VIP）值，對(duì)差異性成分異牡荊素、靛玉紅進(jìn)行定量分析，基于含量測(cè)定結(jié)果建立熵權(quán)逼近理想排序法（technique for order preference by similarity to ideal solution，TOPSIS）-灰色關(guān)聯(lián)度（grey relation analysis，GRA）融合模型進(jìn)一步分析評(píng)價(jià)。結(jié)果 建立的大青葉HPLC指紋圖譜中，識(shí)別共有峰19個(gè)，指認(rèn)峰6個(gè)，分別為峰4（丁香酸）、峰9（阿魏酸）、峰12（異牡荊素）、峰16（色胺酮）、峰18（靛藍(lán)）、峰19（靛玉紅）。指紋圖譜相似度在0.871～0.989；HCA將15批大青葉分為2類；PCA共提取4個(gè)成分，累積方差貢獻(xiàn)率為91.745%；OPLS-DA篩選出異牡荊素、靛玉紅等6個(gè)差異性成分。大青葉中異牡荊素、靛玉紅的質(zhì)量分?jǐn)?shù)在0.781～4.385 mg/g、0.134～0.921 mg/g，熵權(quán)TOPSIS-GRA融合模型結(jié)果顯示15批不同產(chǎn)地大青葉樣品含量存在差異，編號(hào)為S1、S2、S3的樣品質(zhì)量較優(yōu)。結(jié)論 建立的HPLC指紋圖譜和含量測(cè)定結(jié)果可為大青葉的質(zhì)量控制和整體性評(píng)價(jià)提供參考。

Objective The HPLC fingerprint and the index component content determination method were established to evaluate the quality of Daqingye (Isatidis Folium) from different producing areas with chemical pattern recognition methods. Methods Establish HPLC fingerprint of Isatidis Folium, apply the TCM chromatographic fingerprint similarity evaluation system (2012.130723 version), IBM SPSS Statistics 27.0 and SIMCA14.1 software. Combining hierarchical cluster analysis (HCA) and principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA) to evaluate the quality of Isatidis Folium from different producing areas. Quantitative analysis of the differential components, isovitexin and indirubin, was conducted based on the variable importance for projection (VIP) values. Based on the content measurement results, the technique for order preference by similarity to ideal solution (TOPSIS) -grey relation analysis (GRA) fusion model was established to further analyze and evaluate. Results In the established HPLC fingerprint of Isatidis Folium, 19 common peaks were identified, with six peaks assigned, which are peak 4 (syringic acid), peak 9 (ferulic acid), peak 12 (isovitexin), peak 16 (tryptanthrin), peak 18 (indigo), and peak 19 (indirubin). The similarity of the fingerprint spectra ranged from 0.871 to 0.989; HCA classifies 15 batches of Isatidis Folium into two categories; PCA extracts four components with a cumulative variance contribution rate of 91.745%; OPLS-DA identifies six differential components including isovitexin and indirubin. The average mass fraction of isovitexin and indirubin in Isatidis Folium were 0.781—4.385 mg/g and 0.134—0.921 mg/g, respectively. The entropy weight TOPSIS-GRA fusion model shows that there are differences in the content of 15 batches of Isatidis Folium samples from different origins, with samples numbered S1, S2, and S3 having superior quality. Conclusion The established HPLC fingerprint and content determination results can provide a reference for the quality control and holistic evaluation of Isatidis Folium.

R286.2

國家自然科學(xué)基金面上項(xiàng)目（82474099）；大青葉㕮咀飲片研究橫向課題（14207848）