目的 研究堅葉樟Camphora chartophylla醋酸乙酯部位的化學(xué)成分。方法 采用硅膠柱色譜、Sephadex LH-20柱色譜和半制備HPLC等手段進(jìn)行分離純化，通過核磁共振波譜、質(zhì)譜、紅外光譜鑒定化合物的結(jié)構(gòu)。同時，通過MTT和DPPH法評價化合物的細(xì)胞毒性和體外抗氧化作用。結(jié)果 從堅葉樟乙醇提取物中分離得到10個化合物，分別鑒定為(1S,2R)-1-(4-(((1R,2R)-1,3-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-2-yl)oxy)-3-methoxyphenyl)-2-(4-hydroxy-3-methoxy- phenyl)propane-1,3-diol（1）、leptolepisol D（2）、(＋)-(8R,7¢S,8¢R)-9¢-O-(E)-cinnamoyl-5,5¢-dimethoxylariciresinol（3）、(7R,8S)-3,5¢-dimethoxy-4¢,7-epoxy-8,3¢-neolignane-5,9,9¢-triol（4）、2,6,2¢,6¢-tetramethoxy-4,4¢-bis(2,3-epoxy-1-hydroxypropyl) biphenyl（5）、南燭木糖苷（6）、裸柄吊鐘花脂苷（7）、2S-3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol（8）、吐葉醇（9）和桉油烯醇（10）?；衔?b>1～10對RAW264.7細(xì)胞活力無明顯的抑制作用，化合物1、2、5和7顯示一定的抗氧化活性。結(jié)論 化合物1為新化合物，命名為堅木樟素A；化合物1～10為首次從堅葉樟中分離得到，化合物1、2、4、6～8為首次從樟屬中分離得到。化合物1、2、5和7具有抗氧化作用。

Objective To study the chemical composition of acetic ether part of Camphora chartophylla. Methods The compounds were isolated and purified by silica gel column chromatography (CC), Sephadex LH-20 CC, and semi-preparative high-performance liquid chromatography (HPLC). Their structures were elucidated by NMR, MS, and IR spectroscopy. All isolated compounds were subjected to the evaluation of cytotoxity and antioxidant activity in vitro using MTT and DPPH methods. Results Ten compounds were isolated and identified as (1S,2R)-1-(4-(((1R,2R)-1,3-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-2-yl)oxy)-3-methoxyphenyl)-2-(4-hydroxy-3-methoxy- phenyl)propane-1,3-diol (1), leptolepisol D (2), (＋)-(8R,7¢S,8¢R)-9¢-O-(E)-cinnamoyl-5,5¢-dimethoxylariciresinol (3), (7R,8S)-3,5¢-dimethoxy-4¢,7-epoxy-8,3¢-neolignane-5,9,9¢-triol (4), 2,6,2¢,6¢-tetramethoxy-4,4¢-bis(2,3-epoxy-1-hydroxypropyl)biphenyl (5), lyoniside (6), nudiposide (7), 2S-3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol (8), vomifoliol (9) and spathulenol (10). All compounds exhibited no significant inhibitory effect on cell viability and compounds 1, 2, 5, and 7 exhibited certain antioxidant activity. Conclusion Compound 1 is a new compound named chartane A. All compounds are isolated from C. chartophyll for the first time and compounds 1, 2, 4, and 6—8 are isolated from the genus Cinnamomum for the first time. Compounds 1, 2, 5, and 7 exhibited antioxidant activities.

R284.1

國家自然科學(xué)基金項目（82204762）；山東省中醫(yī)藥科技發(fā)展計劃項目（2020M037）山東省中醫(yī)藥高層次人才培育項目；國家中醫(yī)藥管理局科技司-山東省衛(wèi)生健康委員會共建中醫(yī)藥科技項目（GZY-KJS-SD-2023-051）