目的 優(yōu)選鹽沙苑子Astragali Complanati Semen最佳炮制工藝，并對其炮制前后抗炎作用進行研究。方法 在單因素實驗基礎上，采用L₉(3⁴)正交試驗設計，結(jié)合熵權-優(yōu)劣解距離法（逼近理想解排序法，technique for order preference by similarity to ideal solution，TOPSIS），以7個黃酮類成分（山柰酚-3-O-桑布雙糖苷、紫云英苷、沙苑子苷B、沙苑子苷、楊梅素、山柰酚和山柰素）的含量作為評價指標，得到沙苑子最佳鹽炙工藝并進行驗證；建立以2,4,6-三硝基苯磺酸（2,4,6-trinitrobenzenesulfonic acid，TNBS）誘導的斑馬魚腸炎模型，對沙苑子鹽炙前后抗炎作用進行比較。結(jié)果 優(yōu)選得出的沙苑子最佳鹽炙工藝為每30克沙苑子，加2%食鹽水4 mL，悶潤8 h，于100 ℃炒15 min；藥效實驗結(jié)果表明，沙苑子鹽炙后對斑馬魚腸炎模型腸道組織紊亂的改善及降低腫瘤壞死因子-α（tumor necrosis factor-α，TNF-α）、白細胞介素-1β（interleukin-1β，IL-1β）、IL-6和IL-8釋放量等方面略優(yōu)于生品。結(jié)論 熵權-TOPSIS法優(yōu)選得到的鹽沙苑子炮制工藝穩(wěn)定、可行。與模型組相比，鹽沙苑子水提物展現(xiàn)出顯著的抗炎活性，可為闡明鹽沙苑子的炮制增效機制提供參考。

Objective To optimize the best processing technology for salt-processed Shayuanzi (Astragali Complanati Semen, ACS) and investigate its anti-inflammatory effects before and after processing. Methods Based on single-factor experiments, a L₉(3⁴) orthogonal experimental design combined with the technique for order preference by similarity to ideal solution (TOPSIS) method was employed. The content changes of seven flavonoid components (leucoside, astragalin, complanatoside B, complanatoside, myricetin, kaempferol, and kaempferide) were used as evaluation indicators, the optimal salt-stir-frying process was obtained and verified. Additionally, a 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced zebrafish enteritis model was established to compare the anti-inflammatory effects of ACS before and after salt processing. Results The optimal salt-stir-frying process for ACS was determined as follows: for every 30 g of ACS, 4 mL of 2% salt water was added, followed by an 8-hour soak and stir-frying at 100 ℃ for 15 min. The pharmacodynamic experimental results showed that the salt-processed ACS was slightly better than the ACS in improving intestinal tissue disorders in the zebrafish enteritis model and reducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and IL-8. Conclusion The processing technology of salt-processed ACS optimized by the entropy weight TOPSIS method is stable and feasible. The aqueous extract of salt-processed ACS exhibits stronger anti-inflammatory effects than the raw material, providing a reference for elucidating the processing-enhanced mechanism of salt-processed ACS.

R283.6

河北省中醫(yī)藥管理局科研計劃項目（2024096）；河北中醫(yī)學院優(yōu)秀創(chuàng)新團隊項目（YXTD2023007）；河北省“三三三人才工程”資助項目（C2024061）；中藥炮制技術傳承基地建設項目（內(nèi)衛(wèi)辦中（蒙）管理字［2022］278號）