目的 研究裸花紫珠Callicarpa nudiflora地上部分95%乙醇提取物正丁醇部位的化學成分及其體外抗炎活性。方法 采用硅膠、ODS、Sephadex LH-20和高效液相等多種色譜分離技術(shù)進行分離純化，通過理化性質(zhì)和波譜數(shù)據(jù)鑒定化合物結(jié)構(gòu)，新化合物的絕對構(gòu)型則采用電子圓二色譜（electronic circular dichroism，ECD）確定。結(jié)果 從裸花紫珠正丁醇部位分離得到9個化合物，分別鑒定為 (3S,7S)-n-butyl tuberonate-12-O-[6'-O-(E)-feruloyl]-β-D-glucopyranoside（1）、syringaresinol-4'-O-D-monoglucopyranoside（2）、杜仲脂素A（3）、毛蕊花糖苷（4）、異毛蕊花糖苷（5）、木犀草素-7-O-β-D-葡萄糖苷（6）、木犀草素-4'-O-β-D-葡萄糖苷（7）、(7R)-hydroxyeucommic acid n-butyl ester（8）、2-phenylethyl-β-D-glucopyranoside（9）。結(jié)論 化合物1為新化合物，命名為裸花紫珠苷E；化合物8為首次從裸花紫珠分離得到。體外抗炎實驗表明，化合物1、3～5、7～9均有不同程度的抗炎活性。

Objective To investigate the chemical constituents of the n-butanol fraction from the 95% ethanol extracts of Callicarpa nudiflora and evaluate their in vitro anti-inflammatory activity. Methods A variety of chromatographic techniques, including silica gel column chromatography, ODS, Sephadex LH-20, and HPLC, were employed for the isolation and purification of the compounds. Their structures were elucidated based on spectroscopic data and physicochemical properties, while the absolute configurations of the new compound was determined using electronic circular dichroism (ECD). Results A total of nine compounds were isolated from the n-butanol fraction of C. nudiflora, which were identified as (3S,7S)-n-butyl tuberonate-12-O-[6'-O-(E)-feruloyl]-β-D-glucopyranoside (1), syringaresinol-4'-O-D-monoglucopyranoside (2), eucommin A (3), verbascoside (4), isoverbascoside (5), luteolin 7-O-β-D-glucoside (6)、luteolin-4'-O-β-D-glucopyranoside (7), (7R)-hydroxyeucommic acid n-butyl ester (8)、2-phenylethyl-β-D-glucopyranoside (9). Conclusion Compound 1 is a new compound named nudifloside E. Compound 8 was isolated from C. nudiflora for the first time. In vitro anti-inflammatory assays demonstrated that compounds 1, 3—5, 7—9 had anti-inflammatory activities in varying degrees.

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福建省自然科學基金項目（2022J05326）；廣東省自然科學基金項目（2023A1515011761）；深圳市“醫(yī)療衛(wèi)生三名工程”項目資助（SZZYSM202206005）