目的 對(duì)川木香Vladimiria souliei的干燥根的化學(xué)成分及其抗肝損傷作用進(jìn)行研究。方法 運(yùn)用正相硅膠柱色譜、反相ODS柱色譜、Sephadex LH-20、MCI、半制備HPLC、制備TLC、重結(jié)晶等方法對(duì)醋酸乙酯部位進(jìn)行分離純化。采用脂多糖誘導(dǎo)的L02肝細(xì)胞損傷模型，考察化合物對(duì)肝細(xì)胞保護(hù)作用。結(jié)果 得到19個(gè)化合物，包括1個(gè)C₂₁甾體類新化合物 (3β,20S)-pregn-5-ene-3,8,14,20-tetraol-20-O-β-D-glucopyranoside（1）；及18個(gè)已知化合物：erythro-guaiacylglycerol-β-ferulic acid ether（2）、原兒茶酸（3）、3-methoxy-4-hydroxy-phenylethanol（4）、4,5-二咖啡酰基奎寧酸（5）、2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone（6）、松柏醛（7）、芥子醛（mustard aldehyde，8）、1β,6α-dihydroxycostic acid（9）、丁香醛（10）、phenylpropanes coniferyl alcohol（11）、11β,13-dihydroglucozaluzanin C（12）、cynaropicrin（13）、zaluzanin C（14）、11β,13-dihydrozaluzanin C（15）、reynosin（16）、caraumbellogenin（17）、ethyl 3,4-dicaffeoylquinate（18）和baynol C（19）。體外實(shí)驗(yàn)結(jié)果表明，化合物1、11、12和16能顯著逆轉(zhuǎn)脂多糖（lipopolysaccharides，LPS）誘導(dǎo)的L02細(xì)胞存活率下降，且化合物11和12能顯著逆轉(zhuǎn)LPS導(dǎo)致的L02細(xì)胞中丙氨酸氨基轉(zhuǎn)移酶（alanine aminotransferase，ALT）、天門冬氨酸氨基轉(zhuǎn)移酶（aspartate aminotransferase，AST）含量異常升高。結(jié)論 化合物1為新化合物，命名為川木香丁苷；10個(gè)化合物（2～7、9、10、18和19）為首次從該植物中分離得到?；衔?b>11、12具有良好的抗肝損傷作用。

Objective To study the chemical constituents from the ethyl acetate fraction of dry roots of Vladimiria souliei and their anti-hepatocyte injury activity. Methods Multiple kinds of chromatographic methods, including silica gel, ODS, Sephadex LH-20, MCI column chromatography, semi-preparative HPLC, preparative TLC, and recrystallization were used in this investigation. Results A total of 19 compounds were obtained, including a new C₂₁ steroid, named (3β,20S)-pregn-5-ene-3,8,14,20-tetraol-20-O-β-D-glucopyranoside (1) and 18 known compounds erythro-guaiacylglycerol-β-ferulic acid ether (2), catechin (3), 3-methoxy-4-hydroxy-phenylethanol (4), 4,5-dicaffeoyl-quinine acid (5), 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (6), coniferaldehyde (7), mustardaldehyde (8), 1β,6α-dihydroxycostic acid (9), syringaldehyde (10), phenylpropanes coniferyl alcohol (11), 11β,13- dihydroglucozaluzanin C (12), cynaropicrin (13), zaluzanin C (14), 11β,13- dihydrozaluzanin C (15), reynosin (16), caraumbellogenin (17), ethyl 3,4-dicaffeoylquinate (18) and baynol C (19). Among them, compounds 2—7, 9, 10, 18 and 19 were isolated from this plant for the first time. The in vitro experimental results showed that compounds 1, 11, 12, and 16 could significantly reverse the decrease in survival rate of L-02 cells induced by lipopolysaccharides (LPS), and compounds 11 and 12 could significantly reverse the abnormal increase in ALT and AST content in L-02 cells caused by LPS. Conclusion Among the 19 isolated compounds, compound 1 is a new compound named dolomiside D, compounds 11 and 12 have good anti-hepatocyte injury effects.

R284.1

重慶市2021年科衛(wèi)聯(lián)合中醫(yī)藥技術(shù)創(chuàng)新與應(yīng)用發(fā)展項(xiàng)目（2021ZY013997）；西南特色中藥資源國(guó)家重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金（SKLTCM202205）